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Porcine A1-AGP Sandwich Immunoassay Test Kit 96 Wells Size With Strong Sensitivity

Categories Porcine ELISA Kit
Brand Name: BT Lab
Model Number: Cat.No E0063Po
Certification: CE, ISO9001:2015, MSDS
Place of Origin: Shanghai, China
MOQ: Negotiation
Price: Negotiation
Supply Ability: Western Union, T/T
Delivery Time: 1-3 business days, bulk order within one week
Packaging Details: Wrapped with ice pack and styrofoam package
Sensitivity: 16.12mg/L
Standard Curve Range: 30mg/L - 6000mg/L
Delivery: Within 48 hours
Storage: 2-8°C
In Stock: Yes
Customized: Acceptable
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    Porcine A1-AGP Sandwich Immunoassay Test Kit 96 Wells Size With Strong Sensitivity

    Porcine A1-AGP Sandwich Immunoassay Test Kit 96 Wells Size With Strong Sensitivity

    Cat.No E0063Po


    Reagent Provided

    ComponentsQuantity
    Standard Solution (6400mg/L)0.5ml x1
    Pre-coated ELISA Plate12 * 8 well strips x1
    Standard Diluent3ml x1
    Streptavidin-HRP6ml x1
    Stop Solution6ml x1
    Substrate Solution A6ml x1
    Substrate Solution B6ml x1
    Wash Buffer Concentrate (30x)20ml x1
    Biotinylated Porcine A1-AGP Antibody1ml x1
    User Instruction1
    Plate Sealer2 pics
    Zipper bag1 pic

    Material Required But Not Supplied

    • 37°C±0.5°C incubator
    • Absorbent paper
    • Precision pipettes and disposable pipette tips
    • Clean tubes
    • Deionized or distilled water
    • Microplate reader with 450 ± 10nm wavelength filter

    Precautions

    • Prior to use, the Sandwich Immunoassay Test Kit and sample should be warmed naturally to room temperature 30 minutes.
    • This instruction must be strictly followed in the experiment.
    • Once the desired number of strips has been removed, immediately reseal the bag to protect the remain from deterioration. Cover all reagents when not in use.
    • Make sure pipetting order and rate of addition from well-to-well when pipetting reagents.
    • Pipette tips and plate sealer in hand should be clean and disposable to avoid cross-contamination.
    • Avoid using the reagents from different batches together.
    • Substrate solution B is sensitive to light, don’t expose substrate solution B to light for a long time.
    • Stop solution contains acid. Please wear eye, hand and skin protection when using this material. Avoid contact of skin or mucous membranes with kit reagent.
    • The kit should not be used beyond the expiration date.

    Specimen Collection

    Serum Allow serum to clot for 10-20 minutes at room temperature. Centrifuge at 2000-3000 RPM for 20 minutes.


    Plasma Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 15 minutes at 2000-3000 RPM at 2 - 8°C within 30 minutes of collection.


    Urine Collect by sterile tube. Centrifuge at 2000-3000 RPM for approximately 20 minutes. When collecting pleuroperitoneal fluid and cerebrospinal fluid, please follow the procedures above-mentioned.


    Cell Culture Supernatant Collect by sterile tubes when examining secrete components. Centrifuge at 2000-3000 RPM for approximately 20 minutes. Collect the supernatants carefully. When examining the components within the cell, use PBS (pH 7.2-7.4) to dilute cell suspension to the cell concentration of approximately 1 million/ml. Damage cells through repeated freeze-thaw cycles to let out the inside components. Centrifuge at 2000-3000 RPM for approximately 20 minutes.


    Tissue Rinse tissues in PBS (pH 7.4) to remove excess blood thoroughly and weigh before homogenization. Mince tissues and homogenize them in PBS (pH7.4) with a glass homogenizer on ice. Thaw at 2-8°C or freeze at -20°C. Centrifuge at 2000-3000 RPM for approximately 20 minutes.


    Note

    • Sample concentrations should be predicted before being used in the assay. If the sample concentration is not within the range of the standard curve, users must contact us to determine the optimal sample for their particular experiments.
    • Samples to be used within 5 days should be stored at 2-8°C. Samples should be aliquoted or must be stored at -20°C within 1 month or -80°C within 6 months. Avoid repeated freeze thaw cycles.
    • Samples should be brought to room temperature before starting the assay.
    • Centrifuge to collect sample before use.
    • Samples containing NaN3 can’t be tested as it inhibits the activity of Horse Radish Peroxidase (HRP).
    • Collect the supernatants carefully. When sediments occurred during storage, centrifugation should be performed again.
    • Hemolysis can greatly impact the validity of test results. Take care to minimize hemolysis.

    *Sample can't be diluted with this kit. Owing to the the material we use to prepare the kit, the sample matrix interference may falsely depress the specificity and accuracy of the assay.


    Assay Procedure

    1. Prepare all reagents, standard solutions and samples as instructed. Bring all reagents to room temperature before use. The assay is performed at room temperature.

    2. Determine the number of strips required for the assay. Insert the strips in the frames for use. The unused strips should be stored at 2-8°C.

    3. Add 50μl standard to standard well. Note: Don’t add antibody to standard well because the standard solution contains biotinylated antibody.

    4. Add 40μl sample to sample wells and then add 10μl anti-A1-AGP antibody to sample wells, then add 50μl streptavidin-HRP to sample wells and standard wells ( Not blank control well ). Mix well. Cover the plate with a sealer. Incubate 60 minutes at 37°C.

    5. Remove the sealer and wash the plate 5 times with wash buffer. Soak wells with at least 0.35 ml wash buffer for 30 seconds to 1 minute for each wash. For automated washing, aspirate all wells and wash 5 times with wash buffer, overfilling wells with wash buffer. Blot the plate onto paper towels or other absorbent material.

    6. Add 50μl substrate solution A to each well and then add 50μl substrate solution B to each well. Incubate plate covered with a new sealer for 10 minutes at 37°C in the dark.

    7. Add 50μl Stop Solution to each well, the blue color will change into yellow immediately.

    8. Determine the optical density (OD value) of each well immediately using a microplate reader set to 450 nm within 10 minuets after adding the stop solution.


    Summary

    1. Prepare all reagents, samples and standards.

    2. Add sample and ELISA reagent into each well. Incubate for 1 hour at 37°C.

    3. Wash the plate 5 times.

    4. Add substrate solution A and B. Incubate for 10 minutes at 37°C.

    5. Add stop solution and color develops.

    6. Read the OD value within 10 minutes.

    Product Tags:

    elisa test kit

      

    protein elisa kit

      
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