Classic DNA Ladders & Markers 500bp Ladder M1251/M1252

500bp Ladder

Concentration: 92ng/µl

Components

Recommended Loading

3-5 µl/Lane.

Concentration

Typical Bands 100 ng/5µl

Other Bands 40 ng/5µl

Recommended Electrophoresis Condition

5 µl/Lane, 8 cm 1%Agarose Gel, 1×TAE, 7V/cm, 45min

Contents(bp)

500,1000,1500,2000,2500,3000,3500,4000,4500,5000

Storage

1 year at room temperature, for long-time preservation, please storage at -20°C .

Description

500bp ladder is ideal for determining the size of double-stranded DNA from 500 to 5,000 base pairs. The ladder consists of 10 linear double-stranded fragments .The 2,500bp fragment is present at increased intensity to allow easy identification. All fragments are precisely quantified and mixed during the production. For 5 ul loading, all fragments except 2,000bp are 40ng. The 2,000bp fragment is 100ng. This ladder is pre-mixed with loading dye and is ready to use.

Recommendations

Do not heat before loading.

For quantification, adjust the concentration of the sample to equalize

it approximately with the amount of DNA in the nearest band of the ladder.

Visualize DNA by staining with ethidium bromide or with SYBR® Green I.

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Dongsheng Biotech PCR Reagents are looking for distributors and providing OEM service

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Founded in 2005, located in the Science City of Guangzhou, China, Guangzhou Dongsheng Biotech Co., Ltd (GDSBio) is a high-tech enterprise focusing on R&D, production and sales of high-quality life science products and in vitro diagnostic reagents.

GDSBio has a complete product line. With PCR technology as the core, focusing on molecular biology techniques such as ordinary PCR, fluorescence quantitative PCR, NGS library preparation, nucleic acid electrophoresis, nucleic acid purification, etc.It has developed reagents for molecular biology research and diagnosis, and completed the layout of the whole industrial chain from molecular biology reagents, medical lab supplies to specialized services.

Supply Mode: retail/bulk/custom/OEM

With strict and perfect standards, we focus on each product to ensure that every product delivered is in perfect condition.

We provide professional and comprehensive diagnostic and medical test solutions. And we work with clinical experts to continuously improve technology in practice so as to provide more accurate test data for clinical diagnosis.

Since 2010, when GDSBio was certified with ISO9001 and ISO13485 standards in sequence, it has opened up overseas markets. Over the past 18 years, our customers have spread all over the world. With stable and excellent quality, the products have won a large number of loyal customers.

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