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Bullfrog Antigen Rapid Test Kit Colloidal Malachite Green Antigen Test Card

Categories Food Safety Rapid Test Kit
Brand Name: Biokey
Model Number: CG-F001
Certification: ISO13485
Place of Origin: Guangzhou China
MOQ: we produce both liquid and lyophilized kit
Price: USD
Payment Terms: L/C, T/T, Western Union
Supply Ability: 100,000 per day
Delivery Time: depending on order quantity
Packaging Details: Carton Package
Method: colloidal gold
Specimen: Fish, shrimp, bullfrog
Packing: 8T / box
Warranty: 6 months
LOD: 0.5 PPB (µg/kg)
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Bullfrog Antigen Rapid Test Kit Colloidal Malachite Green Antigen Test Card

Malachite Green lateral flow assay diagnostics rapid test card tissue, Aquatic products


Overview:

Malachite Green (MG) rapid detection card is convenient, fast and sensitive, suitable for on-site mass sample detection. The test card contains an antigen in the test area (T) and an antibody ⅱ in the control area (C) which are fixed in advance in the nitrocellulose membrane. The gold label antibody in the micropores was dissolved in the sample and incubated for 3 min. The solution in the gold label micropores was added to the detection card and sample well for 5-8 min reaction. According to T zone, C zone color contrast interpretation results. Whether malachite green was present or not, a purplish red band appeared in zone C.


Detection limit:

Fish, shrimp, bullfrog: 0.5 PPB (µg/kg)


Product component:


Item No.Name
1Malachite Green rapid test card
2Gold micropore (red)
3Extraction agent A
4Extraction agent B
5cleaning agent
6Oxidant
7Buffer solution
810 mL centrifugal tube
95 mL centrifugal tube
10User manual

Preservation and stability:


Store in dry place at 20±5℃, do not freeze, avoid direct sunlight.

This test card is valid for 6 months, and the production date is shown in the packaging box.


Materials required but not provided:


Item No.Name
1Homogenizer
2Vortex Oscillator
3Electronic Balance
4Centrifuge
5Nitrogen (air) blower
6Micropipette


Detection Procedure:


1. Please restore the product to room temperature before use;

2. Open the aluminum foil bag, take the required amount of gold label microholes and test cards, mark them and put them on the table (note: after removing the microholes, seal the aluminum foil bag immediately to prevent moisture);

3. Take the sample to be tested 100 μL into the gold label microhole, blow carefully until the purplish red particles at the bottom of the hole are completely dissolved (note: blow gently to avoid bubbles), and incubate at room temperature for 3 min.

Note: Samples should be tested immediately after processing!

4. Add all the gold label microporous solution into the detection card and sample well, and interpret the result after 5-8 min reaction.


Result Judgment:


Negative: C line color, T line darker than C line color or consistent,The test results were negative.

Positive: C line is colored, T line is lighter than C line or T line is light green, and the test result is positive.

Invalid: Line C does not display color, regardless of whether line T displays color, the detection card is judged to be invalid.


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