Detergent Surfactant CHAPS CAS 75621-03-3 Amphoteric Surfactant

Detergent Surfactant CHAPS CAS 75621-03-3 Amphoteric Surfactant​

Introduction:

CHAPS, a derivative of cholic acid, is a zwitterionic detergent that solubilizes membrane proteins. CHAPS is used to stabilize various protein-DNA complexes and can preserve the biochemical activity of proteins in solution.

Uses:

CHAPS is a non-denaturing zwitterionic detergent used in membrane biochemistry. It can be used to solubilize membrane proteins and disrupt protein-protein interactions. CHAPS has a low micelle molecular weight (6,150) and a high critical micelle concentration (6-10mM), so it can be removed from samples by dialysis. It is also suitable for solubilizing proteins in isoelectric focusing and two-dimensional electrophoresis. CHAPS is commonly used in non-denaturing (urea-free) isoelectric focusing and has been found to give excellent resolution of certain subcellular preparations and plant proteins. Concentrations between 2-4% (w/v) are typically used in isoelectric focusing gels. An amphoteric surfactant, CHAPS is a non-denaturing zwitterionic detergent, protein lysis buffer, used to solubilize membrane proteins and disrupt protein-protein interactions.

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