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| Categories | Peptone |
|---|---|
| Brand Name: | SHIHONG |
| Model Number: | PEPTONE |
| Certification: | SGS |
| Place of Origin: | CHINA |
| MOQ: | 1MT |
| Price: | USD 2.3/KG - USD 3.1$/KG |
| Payment Terms: | L/C, D/A, D/P, T/T |
| Supply Ability: | 2,000MT/MONTH |
| Delivery Time: | 5 WORKING DAYS |
| Packaging Details: | 20KG/BAG 25KG/BAG |
| Company Info. |
| Sichuan Shihong Technology Co.,Ltd |
| Verified Supplier |
| View Contact Details |
| Product List |
Biological Grade Bone Peptone is a specialized nitrogen substrate derived from fresh bovine bone through a controlled enzymatic digestion process. This product serves as an essential nutrient base for industrial fermentation, microbiological culture media preparation, and biopharmaceutical applications, providing readily assimilable nitrogen sources in a highly soluble, low-residue format.
| Processing Phase | Operating Parameters |
|---|---|
| Raw Material Pre-treatment | Fresh bovine bone selection, thermal sanitation, particulate reduction |
| Enzymatic Digestion | Pancreatic enzyme hydrolysis under controlled temperature conditions |
| Primary Separation | Fractionation and initial clarification |
| Purification Sequence | Chromophore removal, odor reduction, membrane filtration |
| Concentration Pathway | Ultrafiltration, thermal concentration, sterile filtration |
| Final Processing | Atomization drying, particle classification, quality verification |
The product exhibits the following functional attributes that support consistent microbial cultivation:
| Characteristic | Description |
|---|---|
| Nitrogen Availability | Dual nitrogen fractions (total and amino) support both primary metabolism and specific biosynthetic pathways |
| Solution Behavior | Complete solubility with thermal stability; no precipitation under standard sterilization conditions |
| Mineral Profile | Controlled sodium chloride and ash content minimize osmotic and inhibitory effects |
| Biological Compatibility | Supports growth of fastidious organisms without supplementation |
| Parameter | Unit | Certified Limit | Analytical Result |
|---|---|---|---|
| Total Nitrogen Fraction | % (w/w) | ≥ 14.5 | 15.2 |
| Amino Nitrogen Fraction | % (w/w) | ≥ 2.5 | 3.1 |
| Sodium Chloride Content | % (w/w) | ≤ 5.0 | 2.56 |
| Residual Moisture | % (w/w) | ≤ 5.0 | 4.5 |
| Mineral Residue (Ash) | % (w/w) | ≤ 5.0 | 3.62 |
| Hydrogen Ion Concentration (2% solution) | pH units | 5.0 – 7.0 | 5.4 |
| Visual Appearance | — | Yellow granular powder | Conforms |
| Aqueous Clarity (2%) | — | Clear solution, no sediment | Conforms |
| Alkaline Stability (pH 8-9, 121°C) | — | No precipitation | Conforms |
| Assay Type | Test Microorganism | Expected Reaction | Observed Performance |
|---|---|---|---|
| Sulfhydryl Amino Acid Detection | Salmonella typhi / Shigella | Positive / Negative | Meets specifications |
| Tryptophan Metabolism | E. coli / S. typhi | Positive / Negative | Meets specifications |
| Glucose Metabolic Pathway (V-P) | Enterobacter aerogenes / E. coli | Positive / Negative | Meets specifications |
| Carbohydrate Utilization | E. coli / S. typhi | No acid formation | Meets specifications |
| Erythrocyte Interaction | S. pneumoniae / β-streptococcus | Alpha / Beta patterns | Maintained through passages |
| Chromophore Expression | P. aeruginosa / S. aureus | Green / Gold pigmentation | Maintained through passages |
| Colony Development | E. coli / S. aureus | Diameter ≥1.0mm, smooth morphology | Meets specifications |
| Minimum Inoculum Response | Streptococcus / Gram-positive bacillus | Growth at 10⁻⁴ / 10⁻⁵ dilution | Meets specifications |
| Sector | Specific Applications |
|---|---|
| Industrial Biotechnology | Microbial enzyme production, organic acid fermentation, single-cell protein cultivation |
| Diagnostic Manufacturing | Dehydrated culture media, selective media formulations, transport media |
| Pharmaceutical Production | Bacterial vaccine manufacturing, toxoid production, fermentor seed cultures |
| Research Institutions | Microbial physiology studies, strain development, media optimization |
| Attribute | Specification |
|---|---|
| Packaging Configuration | 20 kg units with moisture-protective inner liner and durable outer container |
| Storage Parameters | Temperature-controlled environment (≤25°C), relative humidity ≤60% |
| Shelf Stability | 24 months from manufacture date under recommended conditions |
| Material Handling | Minimize atmospheric exposure; reseal containers promptly after use |
Internal Standard: Q/SCSH 001-2025
Reference Document: Chinese Biological Products Main Raw and Auxiliary Materials Quality Control Standard (2000 Edition)
Analytical Methods: GB 5009 series (moisture, ash, nitrogen, pH, sodium chloride) and pharmacopoeial references
Values shown represent lot analysis. Performance characteristics may vary slightly between production batches. Technical consultation available for specialized application requirements.
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